In silico prediction of cross reactive immunologic material (CRIM) status. Variants that leave the translation reading frame intact and that allow utilization of the natural translation initiation codon are predicted to be CRIM positive. This may apply to all types of variants including substitutions, deletions, deletion/insertions, etc., as listed on the HGVS website.
Variants that cause a premature translation termination codon are predicted to be CRIM negative as these likely cause mRNA degradation and loss of protein expression.
Variants that have a predicted effect on pre-mRNA splicing are excluded from the CRIM status prediction as splicing outcome cannot reliably be predicted.